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Genentech inc human cancer cell line rna-seq data (tpm)
(A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public <t>RNA-seq</t> datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).
Human Cancer Cell Line Rna Seq Data (Tpm), supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cancer+cell+line+rna-seq+data+(tpm)/human+cancer+cell+line+rna+seq+data++tpm+/pmc06776997-23-0-7
Average 90 stars, based on 1 article reviews
human cancer cell line rna-seq data (tpm) - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism"

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism

Journal: Developmental cell

doi: 10.1016/j.devcel.2019.07.012

(A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public RNA-seq datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).
Figure Legend Snippet: (A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public RNA-seq datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).

Techniques Used: Expressing, Derivative Assay, RNA Sequencing, Western Blot, Control, Staining, Transduction, Quantitative RT-PCR

KEY RESOURCE TABLE
Figure Legend Snippet: KEY RESOURCE TABLE

Techniques Used: Recombinant, Electron Microscopy, Expressing, Plasmid Preparation

Related Articles

RNA Sequencing:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Expressing:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Derivative Assay:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Western Blot:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Control:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Staining:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Transduction:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Quantitative RT-PCR:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Recombinant:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Electron Microscopy:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Plasmid Preparation:

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism
Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.



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Genentech inc human cancer cell line rna-seq data (tpm)
(A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public <t>RNA-seq</t> datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).
Human Cancer Cell Line Rna Seq Data (Tpm), supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+cancer+cell+line+rna-seq+data+(tpm)/human+cancer+cell+line+rna+seq+data++tpm+/pmc06776997-23-0-7
Average 90 stars, based on 1 article reviews
human cancer cell line rna-seq data (tpm) - by Bioz Stars, 2026-09
90/100 stars
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(A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public RNA-seq datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).

Journal: Developmental cell

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism

doi: 10.1016/j.devcel.2019.07.012

Figure Lengend Snippet: (A) Expression of PTF1A and other acinar regulators in normal human pancreas (n=2) and PDAC cell lines (n=41), derived from public RNA-seq datasets. Expression plotted in transcripts per million, with individual samples plotted in blue over violin plots representing all expressed genes in each sample. (B) Western blot of Ptf1a and tubulin (control) expression in Lenti-TET-Ptf1a derivatives of Panc1, MiaPaCa2, Su8686 and SW1990 cells, treated −/+ DOX (1 μg/ml) for 48 hrs. (C) Representative images from clonogenic growth assays of Panc1 and Su8686 Lenti-TET-Ptf1a cells, grown 2 weeks −/+ DOX and stained with crystal violet. (D) Quantification of relative clone numbers in indicated PDAC cell lines, transduced with Lenti-TET-EGFP (green) or Lenti-TET-Ptf1a (orange), grown 10–14 days −/+ DOX (open vs. closed circles). Distinct lowercase letters indicate p<0.05 from ANOVA followed by Tukey’s post-hoc test of DOX-treated Lenti-TET-Ptf1a cells. (E) Cumulative population doublings of indicated PDAC cells, transduced with EGFP or Ptf1a (green vs orange). Cells were counted and replated at constant densities every 3 days, and treated from day 1 onwards −/+ DOX (open vs. closed circles for individual experiments, dotted vs. solid lines representing means). * P<0.05, DOX-treated Ptf1a vs EGFP cells. (F) Expression of indicated genes in PDAC cell lines transduced with Lenti-TET-Ptf1a, grown 48 hrs −/+ DOX (blue vs. orange), and analyzed by RT-qPCR (ΔΔCt method, normalized to PPIA expression within each sample and untreated Panc1 cells across samples).

Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Techniques: Expressing, Derivative Assay, RNA Sequencing, Western Blot, Control, Staining, Transduction, Quantitative RT-PCR

KEY RESOURCE TABLE

Journal: Developmental cell

Article Title: Prevention and reversion of pancreatic tumorigenesis through a differentiation-based mechanism

doi: 10.1016/j.devcel.2019.07.012

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: Human cancer cell line RNA-seq data (TPM), Genentech , EMBL-EBI Expression Atlas , https://www.ebi.ac.uk/gxa/experiments/E-MTAB-2706/Downloads?ref=aebrowse.

Techniques: Recombinant, Electron Microscopy, Expressing, Plasmid Preparation